AustralieFrV1, Main, Exploration, bibRecord, 00BA14

Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Identifieur interne : 00BA14 ( Main/Exploration ); précédent : 00BA13; suivant : 00BA15

Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Auteurs : Sarah H. D Wilson [Australie] ; Angela M. Bailey [Australie] ; Craig R. Nourse [Australie] ; Marie-Geneviève Mattei [France] ; Jennifer A. Byrne [Australie]

Source :

Genomics [ 0888-7543 ] ; 2001.

RBID : ISTEX:F2F23650EF49362A620CBD2E095599F9BBB8CD32

Descripteurs français

Pascal (Inist)
- Identification, Interaction, Levure, Multigène, Protéine, Protéolipide, Système hybride.
Wicri :
- topic : Droit d'auteur.

English descriptors

KwdEn :
- Academic press, Alonso, Apical, Bait, Bene, Biol, Breast cancer, Breast carcinoma, Breast carcinoma cells, CRHSP28, CSPP28, Carcinoma, Cdna, Cell lines, Chem, Chromosome, Copy number, Copyright, Exocrine secretory granules, Exon, Genbank, Gene family, Genes chromosomes cancer, Genomics, Heterologous, Heterologous binding partners, Heterologous partners, Hf7c, Hf7c cells, Human breast carcinoma, Human chromosome, Hybrid system, Hybridization, Identification, Interaction, MAL, Mal2, Mal2 protein, Mal2 transcript, Mal2 transcript levels, Mal2 transcripts, Membrane microdomains, Multigene family, N8, Novel member, Other sequences, Pancreatic acinar cells, Plasmid, Protein, Proteolipid, Proteolipid family, Puertollano, R10, Skeletal muscle, Solid media, TPD52, Transcript, Transcript levels, Yeast, breast carcinoma.
Teeft :
- Academic press, Alonso, Apical, Bait, Bene, Biol, Breast cancer, Breast carcinoma, Breast carcinoma cells, Carcinoma, Cdna, Cell lines, Chem, Chromosome, Copy number, Copyright, Exocrine secretory granules, Exon, Genbank, Gene family, Genes chromosomes cancer, Genomics, Heterologous, Heterologous binding partners, Heterologous partners, Hf7c, Hf7c cells, Human breast carcinoma, Human chromosome, Hybridization, Mal2, Mal2 protein, Mal2 transcript, Mal2 transcript levels, Mal2 transcripts, Membrane microdomains, Novel member, Other sequences, Pancreatic acinar cells, Plasmid, Protein, Proteolipid, Proteolipid family, Puertollano, Skeletal muscle, Solid media, Transcript, Transcript levels.

Abstract

Abstract: The TPD52 (tumor protein D52)-like proteins are small coiled-coil motif-bearing proteins which were first identified though their expression in human breast carcinoma. TPD52-like proteins are known to interact in hetero-and homomeric fashions, but there are no known heterologous binding partners for these proteins. We now report the cloning of a novel member of the MAL proteolipid family, named MAL2, though its interaction with a TPD52L2 bait in a yeast two-hybrid screen. MAL2 is predicted to be 176 residues (19 kDa) with four transmembrane domains and is 35.8% identical to MAL, a proteolipid required in apical vesicle transport. The MAL2 prey bound all TPD52-like baits tested in the yeast two-hybrid system and in vitro translation of MAL2 produced a single 19-kDa 35S-labeled protein which specifically bound full-length GST–Tpd52 in GST pull-down assays. The gene MAL2, which was localized to human chromosomal band 8q23 and shown to consist of four exons, is predominantly expressed in human kidney, lung, and liver. Our study has therefore identified a novel member of the MAL proteolipid family and potentially implicates TPD52-like proteins in vesicle transport.

Url:

https://api.istex.fr/document/F2F23650EF49362A620CBD2E095599F9BBB8CD32/fulltext/pdf

DOI: 10.1006/geno.2001.6610

Affiliations:

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Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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Pour mettre un lien sur cette page dans le réseau Wicri

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